I 



CYTOCHEMICAL ASPECTS 185 



b c t 



f%* 



, 





■*. 



- 



I 



; -7 



•- 



Fig. 10. Adsorption of purified actin by components of frozen-dried 

 sperm, (a) Transverse and longitudinal sections of sperm exposed to G- 

 actin, 45 min; (b) similar preparation in bovine serum albumin; (c) soaked 

 in deionized water 45 min. After 50 min extraction with 0.6Af KI in 

 0.06M Na 2 S 2 3 ; specimens were treated as previously: (d) G-actin; (e) bo- 

 vine serum albumin: (/) deionized water (x 6,000). 



fied. Moreover, they have been fitted into the organization of the 

 flagellum. The peripheral fibers which contain ATPase, succinic de- 

 hydrogenase and inorganic pyrophosphatase are also reactive with 

 myosin antibodies. Actomyosin extractants abolish reactivity with 

 the antibodies, at the same time removing substances which adsorb 

 extraneous actin. Glycerination washes out IPPase without affecting 

 ATPase or antibody reactivity. Alkaline phosphatase, which may be 



