Fig. 2. Preparation of fish sperm flagella. (a) Electron micrograph of 

 perch sperm flagella. Preparation washed in distilled water and fixed in 

 formaldehyde. Gold-palladium shadowed (x6500). (b) Optical micro- 

 graph of trout sperm flagella prepared in Q.Q3M KC1 buffered to pH 6.8 

 with phosphate (0.0 EU K,HP0 4 , 0.005M KEE_,P0 4 ). In buffer solution, 

 stained with gentian violet (x400). (c) Electron micrograph of the same 

 preparation as (b). Fixed in osmic acid vapor before washing with distilled 

 water. Unshadowed (X6500). 



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