ATPASE AND ACETYLCHOLINESTERASE OF SPERM 249 



Glycerol and digitonin extraction would destroy a control mecha- 

 nism based on permeability changes resulting from acetylcholine re- 

 lease and breakdown; Bishop (1958b) has, in fact, observed that, al- 

 though his sperm models still flagellated on addition of ATP, these 

 waves of flagellation did not progress down the tail and were uncon- 

 trolled. As a result, little translatory motion occurred. 



Acknowledgment. The work which has been described was carried out 

 at Leeds in the Department of Biomolecular Structure. The author would 

 like to acknowledge a real debt of gratitude to the late Professor W. T. 

 Astbury for his encouragement and to his other colleagues for numerous 

 stimulating conversations and for making the years spent at Leeds such 

 happy ones. 



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