18 EXPERIMENTAL EQUIPMENT AND PROCEDURES 



VITAL DYES* 



a. Nile blue sulphate - 1/200,000 



b. Methylene blue - 0. 5% 



c. Neutral red - 1% 



d. Bismarck brown - 1% 



e. Janus green - 1% (see section on "Vital Staining") 



NUCLEAR STAINS 



a. Iron haematoxylin 



b. Feulgen, nucleal reaction 



c. Harris' acid haemalum 



12. Miscellaneous reagents: 



aT Iodine -~s urated iodine in 7 0% (to lollow Zenker's fixation). 



b. Ammoniated alcohol - 5% NH4OH in 70% alcohol for removal of picric. 



c. Glacial acetic acid - and Normal acetic (60 cc. Glacial/liter). 



d. Hydrochloric acid - concentrated, 1% and Normal. 



e. Sodium hydroxide - concentrated, 1%, Normal and 0. 003N. 

 f. Ammo nium hydroxide - concentrated. 



g. Potassium hydroxide - 1%. 



h. Hydrogen peroxide - concentrated (can be used as bleaching agent). 



i. Acetone 



j. Glycerine 



k. Cleaning fluid (K. Bichromate saturated in sulphuric acid). 



1. Clearing agents: 



(T) Xylol (toluene, toluol, benzene (benzol) 



(2) Cedar oil 



(3) Aniline oil 



(4) Oil of wintergreen (methyl salicylate) 



(5) Oil of cloves 



(6) Dioxan (volatile and the fumes are toxic) 



(7) Chloroform 

 m. Mounting media : 



(1) Canada balsam - dissolved in xylol 



(2) Gum damar 



(3) Clarite 



(4) Egg albumen - best as albumen water (3%) 

 n. Embedding media : 



(1) Paraffin: M. P. range from 45°C. to 68 C. 



(2) Beeswax: pure white 



(3) Bayberry wax (Candle Factory, Falmouth, Mass.) 



(4) Rubber - white rubber to be added to paraffin 

 o. Vaseline 



p. pH indicators (LaMotte sets for entire range) 



MISCELLANEOUS EQUIPMENT 



a. Operating base 



(1) Permoplast - American Art Clay Company, Indianapolis, Indiana. 



(2) Rainbow wax - 



(3) Beeswax with lampblack to give proper background shade. 



b. Lampblack 



c. Agar 



d. Cellophane, pliofilm or pyralin. 



* These are concentrated stock solutions. 



