14 EXPERIMENTAL EQUIPMENT AND PROCEDURES 



4. Centriluge - heavy electric centrifuge with large capacity as well has smaller electric 



and hand centrifuges. 



5. Animal cage - a wooden frame with slatted or no floor, screened sides and top, 

 should be made to fit into the water table if the table is large enough. The cage may 

 be divided into compartments with the doors opening on top. If the cage is to sit in 

 water, the wood should be treated with some waterproofing. Frogs and salamanders 

 may be kept in such a cage on the water table in healthy condition if they are provided 

 with cool running water. 



6. Aquaria - the sizes and shapes of aquaria depend upon the particular animals con- 

 cerned. For Urodele and Anuran tadpoles (larvae) the low, flat tanks with consider- 

 able surface are best. Ideal dimensions are the IZ x 12 x 4 enamel (restaurant) pans. 



For fish, four sizes are desirable: 



— for fry - 1/2 gallon battery jars or 10^ store aquaria. 



— for tropicals and breeding pairs -164x81/6x82 inches wide is best (5 



gallons). 



— for larger fish, or large groups of non belligerent fish - 20-4 x 1 O2 x 1 24 



inches high (about 10 gallon capacity). 



— for Zebra fish which are very active - 242 x 62 x 6 inches wide. 



All fish tanks should have slate bottoms and glass covers. One corner of the glass 

 cover may be cut off to facilitate removal, and for feedings. 



The larger tanks may be sub-divided by cutting a piece of glass to fit and then cover- 

 ing the edges of the glass with split rubber tubing and inserting the glass into the 

 tank. The rubber tubing will hold the plate in position and at the same time block the 

 passage of small fry from one compartment to the other. 



7. Microtome - Spencer rotary probably the best. 



8. Embedding ovens - Colunibia probably the best. 



9. . Slide warmer - Chicago Apparatus Conipany, thermostatically controlled electric 



warmer. 



10. Balances - Coarse (200 + gram capacity) and sensitive types. 



GLASSWARE 



1. Battery jars or aquaria lor individual use of students; each with weighted wire screen 

 cover. 



2. Crystallizing dishes or 10 inch finger bowls, with covers. 



3. Finger bowls - regulation size, made to fit into each other. 



4. Petri dishes (10 cm. ) with covers. 



5. Stender dishes with covers, both #1 and #2. 



6. Syracuse dishes - regulation size. 



7. Coplin staining jars. Homeopathic and Shell Vials . 



8. Salt cellars for embedding oven. 



9. Erlenmeyer flasks - 500 cc. capacity for storing sterile media. 



10. Lantern slide covers - used as glass base for operating and for protecting binocular 

 and compound inicroscope stages when using wet mounts. 



11. Graduates - 10 cc. and 100 cc. 



12. Beakers - 100 cc. and 600 cc. 



13. Slides : Regulation microscope slides. 



Depression slides, cell type. 



14. Cover slips - glass. Best size, 7/8 inch square and #1. 



15. Glass rods - Diameters from 4 to 7 mm. Soft glass. 



16. Glass tubing - Diameters from 4 to 7 mm. Soft ^lass. 



