EXPERIMENTAL EQUIPMENT AND PROCEDURES 



part of the rod. Hold the pointed end of the rod in a flame and a glass ball will form 

 on the end. It can be kept symmetrical by constant spinning of the rod while heating. 

 If it is planned to put a gentle curve in such a rod, this should be done when the rod 

 is drawn out rather than later. Such ball-tips are used also for making depressions 

 in Permoplast, suitable for embryos. It is well, therefore, to have a small assort- 

 ment of sizes of such ball-tips. 



6. Glass pipettes : Three types of pipettes will be used: wide-mouthed, micro-pipette, 

 and micro-pipette with lateral control (see diagrams). 



V V \^ 



GLASS BALL TIPS J J 



GLASS TUBING 



SCOEW -iLAMP 



RUBBER TuerwG 



MICRO - BURNER 



MICRO - NEEDLES 



V-V 



HAIR LOOPS 



Z MM OPENING 



FINGER COMTROt 

 Of TERMINAL SUCTION 



@nl 



fr© 



.- SAFETY Pt* 



Tl '^^ PrRALIN 



mANSMRENT BAUCKE 



TRANSFER PIPETTE 



BRUCKE TO HOLD TRANSPLANT IN PLACE 

 ( STULTZ '38) 



The wide-mouthed pipette is used for the transferring of embryos. It is simply a 

 wide bore pipette (6-7 mm. diameter) with a curved and smooth-edged tip. This can 

 be made by cutting off the tip of a medicine dropper and should be available at all 

 times. 



The micro-pipette is made of soft glass with 7-8 mm. outer diameter, pulled out to 

 microscopic dimensions in a manner similar to the making of micro-needles (above). 

 The tip end of such pipettes are often closed but may be trimmed off. These pipettes 

 may be used with ordinary rubber nipple or may be used with rubber tubing and 

 mouth suction. 



The micro-pipette with lateral control has special use in the transfer of isolated 

 pieces of embryos to regions where transplants are desired. It is made in the lollow- 

 ing manner: Pull out some 7-8 mm. soft glass tubing so that the handle portion has 

 a length of about 10 cm. It is better if the capillary end has a slight curve and tapers 

 rapidly. Close the capillary end by melting it in the Bunsen flame and attach a piece 

 of rubber tubing to the larger open end of the pipette. Bring the side of the pipette, 

 at about 3-4 cm. above the capillary end, into a small hot flame. If the capillary end 

 is curved, the point of heating should be on the opposite side of the curve. When this 

 point is soft, blow gently into the rubber tubing and the melted glass will bulge out- 

 ward. The size of the heated area will' determine the size of the bulge. Break off 



