EXPERIMENTAL EQUIPMENT AND PROCEDURES 



GLASSWARE AND CULTURE DISHES 



2 enamel pans 3. 5 x 12 x 24 inches for culturing tadpoles. 



2 battery jars with weighted covers, for living frogs. 



2 crystallizing dishes with covers, 12 inch size, for early stages of amphibian aevelop- 

 ment. 

 12 Syracuse dishes 

 12 #1 Stenders with covers 

 12 #2 Stenders with covers 

 24 regulation finger bowls 



6 Petri dishes with covers 



6 shell depression slides 



1/2 gross microscopic slides 



1 oz. #1 cover slips, square or round. 



2 ft. soft glass tubing, 3 mm. diameter. 

 2 ft. soft glass rod, 4 mm. diameter 



4 ft. soft glass rod, 2 mm. diameter. 



2 hypodermic syringes, 2 cc. cap. , with #18 needles. 



2 glass plates, convenient size 5x7 inches. 



METAL INSTRUMENTS AND EQUIPMENT 



2 watchmaker's forceps, stainless steel #5 



2 adjustable needle holders 



3 scissors: fine pointed to heavy and blunt 

 2 fine, sharp scalpels, one a lancet type 



2 rigid section lifters, two sizes 



2 pairs of forceps, regulation but fine and heavy 



1 triangle file 



1 Bunsen burner with wire gauze and rubber tubing 



MISCELLANEOUS EQUIPMENT 



1/2 lb. Permoplast, cream colored, to be used for operating depressions. 



1/4 lb. beeswax, white, clear 



1/4 lb. soft (low melting point) paraffin 



2 China marking pencils, one blue and the other red. 



1 diamond point or carborundum pencil 



2 needle holders, to hold operating needles of various sizes 



1 plastic cover for needle holder 



2 ft. rubber tubing, 11-12 mm. outside diameter 

 2 ft. rubber tubing 8-9 mm. outside diameter 



THE PREPARATION OF INSTRUMENTS 



1. The Micro-burner : This may be set up for class use. However, its preparation may 

 be described since individuals may prefer to have accessible their own burner. Its 

 function is to give a small but intense flame. 



Secure a 5-inch piece of 7 mm. soft glass tubing and in a hot flame reduce the diam- 

 eter of one end to about 1 mm. Then place a right angle in the tube, at its center 

 (with a flame tip) and when cool, pass the flamed end through a hole in a large cork. 

 Mount the cork in a burette clamp attached to a ring stand so that the micro-burner 

 is turned upward. To the other end of the tube attach a rubber tubing joined to the 

 gas outlet. Apply a screw clamp to the rubber tubing. By regulating the screw clamp 



