33. Extirpation Experiments on Organ Anlagen 250 



34. Transplantations 257 



35. The Origin of Amphibian Pigment 265 



E. FIELD OPERATIONS 



36. Limb Field Operations 273 



37. Eye Field Operations 283 



38. Heart Field Operations 297 



39. Regeneration 305 



F. ENDOCRINE FACTORS IN DEVELOPMENT 



40. Effect of Thyroid and of Iodine on Amphibian Metamorphosis 315 



41. Thyroidectomy and Early Amphibian Development 320 



42. Hypophysectomy and Early Amphibian Development 322 



G. CHEMICAL EMBRYOLOGY 



43. Cytochemical Tests for Gametes and Embryos of Amphibia 325 



III. EXPERIMENTAL FISH EMBRYOLOGY 



44. The Care and Feeding of Laboratory Fish 345 



45. Fish Diseases 351 



46. Fish Suitable for Laboratory Experimentation 354 



a. Egg-layers, 354 f. The Sea-horse, Hippocampus Hudsonius, 360 



b. Live Bearers, 355 g. The Japanese Medaka, Oryzias Latipes, 360 



c. Breeding of the Betta, 355 h. The Platy, Platypoecilus Maculatus, 369 



d. The Zebra Fish, Brachydanio Rerio, 357 i. The Normal Teleost: Fundulus Heteroclitus, 375 



e. The Jewel Fish, Hemichromis, 359 



47. Experimental Procedures with Fish Eggs and Embryos 384 



a. Vital Staining of Fish Embryos, 385 



b. Injury, Ablation and Recovery of Fish Embryos, 387 



c. The Culturing of Fish Explants in Vitro, 391 



d. Induction of Secondary Embryo by Grafting of Dorsal Lip, 394 



48. The Genetics of Fish 397 



IV. EXPERIMENTAL CHICK EMBRYOLOGY 



49. Procuring and Care of Living Material 405 



50. Technical and Operating Equipment 407 



51. The Removal of Chick Blastoderms or Embryos 409 



52. Histological Procedures 415 



53. Methods for Observing the Development of the Chick Embryo 418 



54. Morphogenetic Movements Determined by Vital Staining and Charcoal 



Particles 422 



55. Explanting and Cultivating Early Chick Embryos in Vitro 428 



56. The Method of Chorio-AUantoic Grafting 437 



57. Intra-Embryonic Transplantations 443 



V. EXPERIMENTAL MAMMALIAN EMBRYOLOGY 

 Suggested Procedures for Obtaining and Experimenting with the Mouse Embryo . . 449 



• 



GLOSSARY OF TERMS IN EXPERIMENTAL EMBRYOLOGY 'i^'? 



INDEX 499 



