EXPERIMENTAL EQUffMENT AND PROCEDURES 



29 



PHOTOGPaPHIC FOR'.'LLAE 



DISSOLVE IN THE ORDER 



I ALL WEIGHTS IN GRAMS 



GIVEN I 



rDEVEr0PIN?TIMr'^R'TSTEs'7?lD'^U»l?TT'T5^ 



ACID HARDENING FIXING BATH FOR RftPERS 

 F- I 



WATER I UTER 



HYPO 240 GMS 



WCN THOROUGHLY DISSOLVED ADO SLOWU' WHILE 

 STIRRING THE FOLLOWING HARDENING SOLUTION TO 

 THE COOL MTT>0 SOLUTION, 



WftTER (125* n 

 SOOIUM SUL/ITE 

 ACETtC AClO. 26 '* PURE 



8 CC. 



I 5 6M& 



4 B C-C. 



I 5 GMS 



CLEARING SOLUTION FOR 

 LANTERN SUDE PLATES 



POT &I.UM 

 CITRIC AOO 



WATER 



CHROME ALUM HARDENER 



WATER 



POT CHROM ALUN 

 ACETIC AGIO 

 WATER TO 



500 CC 



1 5 GMS 



2 2 GMS 

 I LITER 



PLATES AND FILMS' 



DILUTIONS 



EDWAL 12 

 EDWAL 20 

 COPY 



NONE 

 NONE 

 NONE 

 NONE 



NOrc 



l-I 

 A (tl 6 



PAPERS 



BROM. 



CONT 



FOR MORE CONTRAST 



00 NOT AGITATE PAPER IN 



DEVELOPER 



IT^^n^orawinS^^^^I 

 COPY - FOR OflTHO PLATE I 

 PHOTO - DETAIL | 



MERCURY INTENSIFIER 



27. S GMS 

 1000 CC 



RMSE IN 

 KYDflOCH.ORC AOD 



REDEVELOP IN MQ DEVEXOPER 



REFERENCES 



ADAMS, A. E. , 1928 - "Paraffin sections of tissue supra -vitally stained. " Sc. 68:303. 



BACON R. L. , 1947 - "The adaptation of block surface staining of fetuses embedded in ethyl methacrylate. " Anat. Rec. 99. 

 BASSETT. D. L. , 1947 - "Ethyl methacrylate as a preserving medium for gross anatomical serial sections. " Anat. Rec. 99. 

 BECKER, R F. , 1940 - "Experimental analysis of Kuo-Vaseline technique for studying behavior development in chick embryos. 



Proc. Soc. Exp. Biol. G Med. 45:689. 

 BELAR, K. , 1928 - "Methodik der wissenschaftlichen Biologie. " ed. by T. Peterfi, Vol. 1:779. 

 BRACHET, J., 1947 - "Embryologic Chimigue" Mosson et Cie. , Paris. 

 BRADBURY, F. R. GD O. Jordon, 1942 - "The surface behavior of anti-bacterial substances. I. Sulfanilamide and related 



substances. " Bioch. Jour. 36:287. 

 BRAGG, AN, 1938 - "The organization of the early embryo fo Bufo cognatus as revealed especially by the mitotic index. " 



Zeit. f. Zellforsch. u. mikr. Anat. 28:154. 

 COLE, E C, 1946 - "Improved fixation in vitally stained methVlene blue preparations. " Stain Tech. 21:163. 

 COPENHAVER, W. M. , 1939 - "Initiation of beat and intrinsic contraction rates in the different parts of the Amboystoma heart. 



Jour. Exp. Zool. 80:192. 

 DAVIDSON, M. H. , 1945 - "The preparation of frog embryology slides. " Turtox News. 23:33. 

 DAW/SON, A B. , 1939 - "Visualization of the vertebrate skeleton in the entire specimen by clearing and selective staining. " 



Am- Biol. Teacher. 1:91. 

 DAWSON, A B , 1939 - "Differential staining of the anterior pituitary of the cat. " Stain Tech. 14:133. 



