146 



TEMPERATURE INDUCED HETEROPLOIDY 



Fix the tail tip in Bouin's fluid (it is not necessary to kill the larva) after clipping it off 

 about 1/3 the distance from the tip to the base of the body. After about 4 hours, wash in 

 the usual manner (decolorize in 0. 5% NH^OH), wash in running tap water to neutralize 

 the tissue, and then stain for 7 to 1 minutes in about 33% Harris' acid haemalum (freshly 

 acidified). Blue in tap water (alkaline), then dehydrate in the usual manner, mount, and 

 study. The percentage of cells showing chromosome figures will be small, best in 

 Triturus viridescens and poorest in the Axolotl and Rana. Drawings and photographs of 

 chromosome groups constitute the record, in addition to which drawings and photographs 

 of haploid, diploid, and triploid larvae, their chromatophores and relative cell sizes 

 would be convincing (See figures on following page). 



DIRECT EVIDENCE OF HAPLOIDY 



Diagrammatic drawing of the metaphase chromosomes of a dividing epidermal cell 

 from the amputated tail tip of 19-day haploid larva. The haploid chromosome number 

 in this species is twelve; normal diploid tissue having twelve pairs of chromosomes. 



(Triturus pyrrhogaster) 



From Fanl<hauser 1937; Jour. Heredity 28:1 



ABNORMALITIES FOUND IN HAPLOID 

 EMBRYOS AND LARVAE OF AMPHIBIANS 



Table from Fankhauser 1945: Quart. Rev. Biol. 20:20 



