212 



VITAL STAINING AND MORPHOGENETIC MOVEMENTS 



C PROSPECTIVE POTENCY D REGION OF PRIMARY INDUCTOR 

 (0RGANI2ER) 



A. profile view of the early gastrulo showing the 

 prospective areas as revealed by the vital staining 

 method. B. similar view showing power of self- 

 differentiation of small pieces when isolated. 



C. showing prospective potency (powers of dif- 

 ferentiation under various tried conditions). 



D. showing the primary inductor area; stippled 

 area, head organizer; area with dashes, trunk- 

 tail organizer. After Holtfreter (1936). 



(From Harrison 1945: Trans. Conn. 

 Acad. Arts & Sci. 36:277) 



iJAUO ANTC»10«V1£« 



STACCS. Kn&M.tf<« 



The relative position of the presumptive ecto- 

 dermal organ rudiments of the head in early 

 neurula stages of Amblystoma punctatum. 



(From Carpenter 1937: Jour. 

 Exp. Zool. 75:103) 



REFERENCES 



ADAMS, A. E. , 1928 - "Paraffin sections of tissue supravitally stained. " Science, 68:303. 



BANKI, O. , 1927 - "Die Lagebeziehungen due Spermium-Eintrittsstelle zur Medianebene und zur ersten Furche, nach Versuchen 

 mit orlichen Vitalfarbungen Axolotei. " Anat. Anz. 63:198. 



CARPENTER, E. , 1937 - "The head pattern in Amblystoma studied by vital staining and transplantation methods. " Jour. Exp. 

 Zool. 75:103. 



CONN, H. J. G R. S. CUNNINGHAM, 1932 - "The use of dyes as vital stains. " Stain Technology. 7:81. 



CUNNINGHAM, R. S. G H. J. CONN, 1932 - "Methods for the preservation of supravitally stained material. " Stain Technology. 

 7:15. 



DETWILER, S. R., 1937 - "Application of vital dyes to the study of sheath cell origin. " Proc. Soc. Exp. Biol. C Med. 37:380 

 (See Detwiler, 1917, Anat. Rcc. 13:493). 



FRANKSTON, Jane E. , 1940 - "The photodynamic action of neutral red on Triturus viridescens. " Jour. Exp. Zool. 83:161. 



GOERTTLER, K., 1925 - "Die Formbildung der Medullaranlage bei Urodelen. Im Rahman der Verscheibungsvorgange von 

 Keimbezirken wahrend der Gastrulation und als entwicklungs-physiologisches Problem. " Arch. f. Ent. mech. 106:503. 



HAMBURGER, V., 1960 - "Description of Gastrulation in Urodeles. " in "A Manual of Experimental Embryology." p. 47, 

 Univ. Chicago Press. 



HARVEY, E. B., 1941 - "Vital staining of the centrifuged Arbacia punctulata eggs. " Biol. Bull. 80:114. 



PASTEELS, J. , 1942 - "New observations concerning the maps of presumptive areas of the young Amphibian gastrula (Amblystoma 

 and Discoglassus). " Jour. Exp. Zool. 289:255. 



STONE, L. S. , 1933 - "The development of lateral line sense organs in amphibians observed in living and vital stained prepara- 

 tions. " Jour. Comp. Ncur. 57:507. 



VOCT, W., 1929 - "Gestaltungsanalyse am Amphibienkeim mit ortlicher Vitalfarbung. II. Gastrulation und Mesodermbildung 

 bei Urodelen und Anuran. " Arch. f. Ent. mech. 120:384. 



VOTQUENNE, M. , 1934 - "Experiences de Destniction des Micromcrcs dorsaux dc I'oeuf de Rana fusca stad VIII, et interpreta- 

 tion des Resultats par la Methode des Colorations vitales localisees. " Arch, de Biol. 45:80. 



ZORZOLI, A., 1946 - "Effects of vital dyes on the early development ot the amphibian embryo. " Proc. Soc. Exp. Biol. G Med. 

 63:565. 



