232 



TISSUE CULTURE TECHNIQUES 



Break eqg into BSS in 

 a crystallization dish 



Carefully dissect out 

 blastoderm. 



With a wide bore pipette 

 transfer blastoderm to 

 petn dish Separate 

 vitelline membrane 



Transfer embryo to 

 culture dish Remove 

 excess fluid 



Under the dissectinq 

 microscope arranqe 

 the embryo ventral side 

 down 



Culture of the whole chick embryo 



Mark large vessels 



Cut window. 



Remove window 

 with needle 



Place drop of saline 

 over vessels 

 Puncture air space 

 Open shell membrane. 



Place tissue 

 in position. 



Seal with 

 Sellotape. 



Cultivation of tissue on the chorio-allontoic membrane of the fertile egg 



(Figures reproduced with permission from 

 J. Paul's "Cell and Tissue Culture" 1960, 

 E. C S. Livingston, Pub., Edinburgh.) 



