TRANSPLANTATIONS 



259 



OLFACTORY PIT 



SUCKER 



EXTERNAL 

 GILLS 



LATERAL VIEW 



DORSAL VIEW 



1. Select two embryos, Anura or Urodela, of the same age and size and place them in 

 Standard Solution in a Permoplast or paraffin operating Syracuse dish. Prepare shal- 

 low depressions to hold them right side uppermost. Remove all the membranes. 



2. Prepare the host site by removing a piece of ectoderm and underlying mesoderm from 

 different sites on different embryos, e. g. , just posterior to the normal position of 

 the gill anlage'; beneath somites 9 to 12; just posterior to the anterior limb bud; just 

 anterior to the posterior limb bud; or in the position of the eye. Excavate deeply 

 enough to provide adequate room for a transplant with considerable thickness. 



Amblystoma tigrinum gill 

 anlage transplanted to the 

 belly region of A. punctatum. 



