INDEX 



Abnormalities in development, 76-81 



Acris gryllus, 32 



Age (of egg), 125 



Ambly stoma jeffersonianum, 41 



Amblystoma opacum, 42 



Amblystoma punctatum, 43, 82-87 



Amblystoma tigrinum, 43, 44 



Amphibia, breeding habits, 31 



Androgenesis, 178-182 



Anesthesia, 165-169 



Anesthetics, 16, 20 



Animals source, 19, 20 



Anlage' (primordium), 206. 207, 218, 250 



Asepsis, 1, 11, 12 



Azure A-Phthalate stain (nucleic acids), 328 



Bacteria, 53 

 Balancers, 251, 261 



Behaviour, 111; (of isolated cells), 213 

 .Betta (fish), 355-357 

 Biologically clean, 1 

 Blastulation, 210 

 Bleaching, 23 



Brachydanio rerio (see Zebra fish) 

 Brain, 254 



Breeding, induced, 91, 97 

 Bridge, glass, 10 

 Bufo americanus, 38-40 

 Bufo fowleri, 39, 40 

 Burner, micro, 7 



Cell adhesiveness, 214 



Cell fragmentation, 214 



Cell motility, 213 



Cells, dissociation of, 224 



Cells, reaggregation of, 224 



Centrifugation, effect on cleavage, 187-192 



Chemical effect on growth and differentiation, 



162-164 

 Chick embryo, care of, 405 

 Chick embryo, culture medium for, 407, 428- 



433 

 Chick embryo, culturing, 430-433 

 Chick embryo, experimental, 405-448 

 Chick embryo, histological procedures, 415- 



417 

 Chick embryo, observations on development, 



418-421 

 Chick embryo, operational procedures, 407 

 Chorio-allantoic grafting, 439-442 

 Chromosomes, 128, 132, 144, 172 

 Clearing, 24 

 Control, 3 



Culture of anlagen, 218, 266, 301 

 Culture medium, 49 (see under generic name) 

 Culturing, fish explants in vitro, 391 

 Culturing, chick embryo parts, 428-436 

 Cyclopia, 293 

 Cytochemistry, 325-344 



Dehydration, 23 



Development, effects of centrifugation, 189-192 



Development, effects of hypophysectomy, 322- 



3 24 

 Development, effect of thyroid on, 320-322 

 Developmental stages, 56 (see under specific 



forms) 

 Diemyctlylus viridescens (see Triturus virides- 



cens) 

 Differentiation, 149, 158-164, 216 

 Dorsal lip of blastopore: amphibia, 198, fish, 



394 

 Dyes, vital, 18 



Egg transport, 104-108 

 Equipment needed by student, 5 

 Equipment, standard laboratory, 13, 18, 19 

 Equipment supply companies, 19 

 Embedding, 24 



Embryos, production of double, 193-196 

 Enzyme tests, 339-340 

 Estrous, mouse, 463 

 Eurycea bislineata, 44 

 Evocation, 202 

 Experimental procedure, 3 

 Experimental program, 2 

 Explant, 219: chick, 428-436 

 Extirpation experiments, 250 



Eye field, 251, 253, 261, 283-296: chick trans- 

 plant, 444 



Fertilization, artificial, 94, 99, 100, 109, 116 



Feulgen reaction, 326 



Fish, care and breeding of, 345-404 



Fish diseases, 351-353 



Fish, egg layers, 354 



Fish for experimentation, 354-396 



Fish genetics, 397-404 



Fish, live bearers, 355 



Fixatives. 16, 17, 21-23 (see under specific 



names) 

 Food, anura, 51, 52 

 Food, larvae, 51 



Food, post-metamorphosis, 51, 52 

 Food, urodela, 51, 52 

 Form, effect of narcosis on, 165-169 

 Frogs, 32 (see under generic names) 

 Function, effect of narcosis on, 165-169 

 Fundulus heteroclitus, 375-383 



Gastrulation movements (frontispiece), 207 



Genetic factors in limb formation, 278 



Genetics of fish, 397-404 



Germinal vesicle, 170-177 



Gills, external, 251, 252, 258 



Glass ball tip, 8 



Glass pipette, 9 



Glassware, 11, 14 



Glycogen tests, 339 



- 499. 



