50. TECHNICAL AND 

 OPERATING EQUIPMENT 



Provided by the Institution: 



Temperature-controlled incubator(s) with turning and hatching trays. 



Autoclave 



Dry sterilizing oven 



Heating plates or electrically-controlled stage warmers for warming eggs or em- 

 bryos during operations. 



Candling equipment 



100 cc. beakers with (soft) paraffin and small (paint-type) brushes 



Absorbent cotton 



Covered containers for discarded eggs 



Spencer microscope lamp, condensor and diaphragm. 



Dissecting microscope 



Round bottom (250 cc. ) flask of water supported by double pinch-clamp, to absorb 

 heat from light source. 



Solutions : 



Glass distilled water 



Locke's (1907 Jour. Physiol. 36:208) solution 



NaCl - 0.9gr. NaHCOj - 0. 02 gr. 



KCl - 0. 04 gr. Dist. water - 100 cc. 



CaCl2 - 0. 024 gr. (anhydrous) (Use at 38° - 40° C.) 



Romanoff's Isotonic Avian Ringer's Phosphate Buffer : 



1. Measure out 5 cc of concentrated (x20) avian Ringer's 



NaCl 8. 6 gms. 



KCl 0. 31 gms. 



CaCl2 0. 20 gms. 



MgCl- 2H2O 0. 10 gms. 



2. Add 70 cc of distilled water. 



3. Add 0. 2 gms. maltose (powdered, anhydrous) 



4. Add slowly 10 cc of primary and secondary phosphate buffer solutions: 



NaoHPO^; M/15 800 cc. , „„„ 



KH2P04. M/15 200 cc. ?" ^°°°"- 



Make up to 100 cc. with distilled water. 

 This medium has a pH of 7. 3m. , but by standing overnight this can change 

 as much as to pH 6. 9. It was first described in 1943 (Jour. Exp. Zool. 

 93:1-26) by Dr. Alexis Romanoff, but the above modification has never been 

 published although it is in common use in Dr. Romanoff's laboratory. 

 Physiological saline (0.9% NaCl) 

 Phenol red (of known concentration) 



MS 222, made up 1/3, 000 in Locke's of physiological saline (anesthetic). 

 Fixatives: Bouin, Kleinberg' s Picro- sulphuric, Aceto-formalin. 



Provided by the Student: 



12 petri dishes (4" diameter) 

 12 watch crystals (2" diameter) 



2 regulation finger bowls 



2 Erlenmeyer flasks (500 cc. ) 



2 Erlenmeyer flasks (125 cc. ) 



1 graduated cylinder (100 cc. ) 



1 graduated cylinder (10 cc. ) 



2 wide-mouthed pipettes, smooth edges (inside diameter 4 mm.) 



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