' l0 M '". 



Figure 1 1 . Spectrogram 

 in the Soret band region 

 of a cross section of a 

 frog red blood cell, 

 showing Soret band 

 absorption strong in the 

 cytoplasm and absent 

 in the nucleus. 





/^\A-\436m / , 



,- r ' ^v 



405 m 



y 



■■y 



■ ^ 



in 



Figure 12. Micro- 

 photometer records 

 at various wave- 

 lengths across the 

 spectrogram of a 

 frog red blood cell 

 obtained from a 

 reflecting micro- 

 scope and quartz 

 spectrograph. 



-Cytoplasm 

 -Nucleus 



~ """" s_ * -" T - 

 ' ■ ■: 



_ 312 • u 

 297 -l 



-Cytoplasm 



-Sector 



A 266 



312 



I I I 



366 405 436 tll/l 



Figure 13. Spectrogram 

 of a frog red cell using a 

 mercury arc (three dif- 

 ferent exposure times) 

 with a rotating sector in 

 the lower part of the field 

 for density calibration. 

 This spectrogram is a 

 negative, i.e. greater 

 black ening represents 

 greater transmission of 

 the object. 



06 



OS 



0-4 



x 

 a rj 



c 2 



3 o 



%os 



Rotating Sector 



40S m/i 



Cytoplasm 



m 



Figure 14. Photometer records across a 



spectrogram of a frog red blood cell, with 



rotating sector for density calibration in the 



lower part of the field. 



C 2 

 0-1 







Cytoplasm 



