Osmotic Pressure of Haemoglobin 



which show a graphic representation of the data obtained from 

 osmotic pressure measurements on solutions of horse and human 

 haemoglobin respectively in 0-2 M phosphate, demonstrate the order 

 of accuracy and type of plot obtained. The slope of the horse haemo- 

 globin plot is the same as that obtained by plotting Adair's data for 

 sheep haemoglobin on the same scale 5 . The slope of the human 

 haemoglobin plot is slightly larger, in agreement with some data given 

 by G. S. Adair 4 . 



Plot ofPjC against C, where C 

 is the concentration of haemo- 

 globin in grams per 100 ml of 

 solvent and P is the osmotic 

 pressure in cms of water, a 

 Horse haemoglobin solutions in 

 0-2 M phosphate, b Human 

 haemoglobin solutions in 0-2 M 

 phosphate, c Horse haemo- 

 globin (O) and human haemo- 

 globin (®) solutions in 0-2 M 

 phosphate + 1 M sodium 

 chloride, d Horse haemoglobin 

 solutions in 2 M sodium chloride. 



0~ 



b 



5 6 

 C 



10 II 



Table III 



Table IV 



The Osmotic Pressure of Solutions 



of Horse Haemoglobin in 0-2M 



Phosphate and \M NaCl 



The Osmotic Pressure of Solutions 



of Human Haemoglobin in 0-2M 



Phosphate and \M NaCl 



201 



