h. m. jope and J. r. p. o'brien 



then transferred to small test tubes, containing a glass bead to 

 facilitate mixing, which were filled with CO, O a or N 2 for HbCO, 

 Hb0 2 and Hb, and tightly closed. The tubes were kept at constant 

 temperature and were rotated gently by hand at intervals for a period 

 of about 2 days, by which time constant values had usually been 

 reached and remained so for a further week. 



HbCO or MHb Added, ym% 

 10 20 JO ' 40 



10 20 



HbCO Added, gm% 



Figure 9. Solubilities of human 

 carboxyhaemoglobin and 

 methaemoglobin related to total 

 haemoglobin present in liquid 

 and solid phases. 



In Figure 9 the concentration of HbCO in solution in potassium 

 phosphate buffer pH 6-7, 1M at 0°C (curve 1) and 2M at 22-5°C 

 (curve 2) is plotted against increasing quantities of solid phase. Data 

 are also given for MetHb in 1M phosphate (curve 1). In 2M phos- 

 phate the curve is linear until a constant solubility is reached at 

 17 per cent HbCO. 



?- 2 



Figure 10. Variation of log \ 



solubility of human adult haemo- ^ 



globins with ionic strength of % i 



phosphate buffers pH 6*7. | 



£>p 



272 



