Fig. 49 

 Control tissue spread, rat subcutaneous connective tissue. Toluidine blue ( 320). 







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Fig. 50 



Tissue spread, rat subcutaneous connective tissue following vigorous shaking in 25 per cent 



sucrose for two hours. Only a few scattered granules remain at the site of the mast cells. 



Note also that the endothelial nuclei now stain with toluidine blue ( x 320). 



I 



129 



