SPECIFIC AND NONSPECIFIC RESISTANCE 



Mortality ratios at lU days 

 Temperature Treatment (grouped) P(x2) 



210 C 



Normal 

 Immune 

 Zymosan 

 Endotoxin 



15/20n 

 0/20 

 8/20-J 



1 



11/19 _l_ 



~I 



0.05 - 0.02 

 ■0.3 - 0.2 

 -0.5 - 0.3 



2© C 



Normal 

 Immune 

 Zymosan 

 Endotoxin 



17/20 



0/20 



9/20_J 

 m/20 



0/10 



0.02 - 0.01 

 0.3 - 0.2 

 0.2 - 0.1 



Table XI. Effect of acute coUi stress (2° C) on mortality of mice challenged intra- 

 peritoneally with 10 LD5Q* of Klebsiella pneumoniae. Unacclimatized animals immunized 

 or treated and challenged at room temperatures, then placed at noted temperatures. 

 ♦10 LDgQ = 1000 organisms per mouse. 



ten. The results are shown in Tables X and XI. Table X shows that 

 mice specifically immunized to the challenge agent, S. aureus, were 

 significantly protected even though acutely exposed to 2° C. The 

 mortality ratios of mice treated with zymosan or endotoxin prior to 

 challenge was considerably increased over the specifically immu- 

 nized group. The results indicate that the increased resistance af- 

 forded mice by specific immunization was not depressed by acute 

 exposure of the animals to 2° C. The resistance induced, then, ap- 

 peared to be temperature independent. There were no singly- caged 

 animals in this experiment, since it was apparent from prior re- 

 sults that grouping afforded maximal protection even in groups with 

 as few as three animals. 



In Table XI are summarized the results of acute cold stress of 

 specifically and nonspecifically immunized animals challenged with 

 ten LDgo's of K. pneumoniae . By increasing both the animal group 

 size and challenge inoculum dose, we felt that the data obtained would 



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