SPECIFIC AND NONSPECIFIC RESISTANCE 



Temperatures Treatment Mortality ratios at IH days 



(grouped) (single) 



Normal 3/10 



Immune 0/10 



210 C 



Zymosan 0/10 



Endotoxin 2/9 



Normal U/IO 6/10 



Immune 0/10 2/10 



2« C Zymosan 0/10 9/10 



Endotoxin 9/10 7/10 



0/10 0/10 



Table IX. Effect of chronic cold stress (2° C) on mortality of mice challenged intra- 

 peritoneally with 1 LD^q* of Klebsiella pneumoniae. Animals maintained for 30 days, 

 immunized or treated and challenged at noted temperatures.*! LDqq of K. pneumoniae = 

 68 organisms per mouse, 



or as individuals. The nonspecific immunization treatment did not 

 result in significant protection compared to the controls or to the 

 specifically immunized animals. The mortality ratio of the zymosan 

 treated animals compared to the endotoxin treated animals is not 

 significantly different. When comparing mortality results of animals 

 that are acclimatized versus the acutely cold stressed animals 

 (Table VI vs. Table VIII) , note that the acclimatized animals that 

 are specifically immunized did well grouped or caged individually. 

 In contrast, the acutely cold stressed singly- caged mice were not 

 benefited by specific immunization to the extent noted for the acutely 

 stressed mice caged in groups. 



When the cold acclimatized mice were challenged with one 

 LD5Q of K. pneumoniae (Table IX) , both specific immunization and 

 zymosan treatment were beneficial to animals kept at 21 C or in 

 groups at 2° C. However, protective effects of endotoxin were 

 not apparent under these conditions. When animals that have been 



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