SPECIFIC AND NONSPECIFIC RESISTANCE 



MIYA: Yes, we removed the peritoneal exudate, pooled the 

 samples, and reinjected this material into another group of 

 animals. 



MARCUS: And the data shown from the isolates would be or- 

 ganisms from the seventh pool. 



REINHARD: How many determinations did you make of the 

 oxygen requirements; how many parallel experiments did you 

 run? 



MIYA: Each Warburg flask was set up in duplicate, and the 

 runs were done three times. 



REINHARD: Isn't it possible you could have run into chance 

 variations? I would be more comfortable if you said you ran 

 fifty determinations. 



MIYA: I could have shown a series of curves that would es- 

 sentially show what I have shown here. I chose, rather than to 

 confuse the issue, to show a group of typical curves obtained 

 for any one determination, 



REINHARD: Then how did you test these organisms after they 

 had grown out again to see whether they recaptured their ori- 

 ginal state or whether they remained the same in their oxygen 

 requirements ? 



MIYA: I don't think I quite understand. 



NUNGESTER: He means, if they're carried along as a pure 

 line culture, how long do they retain this altered characteristic? 



MIYA: After the seventh passage, we did the oxygen uptake 

 studies and we didn't do any more after that. We merely saved 

 those organisms for the challenge in subsequent experiments. 



WALKER: I'd just like to comment on this curious, inter- 

 estir^ difference in the effect of cold on various strains of mice 

 that Dr. Miraglia mentioned. In our strain of Wisconsin mice, 



20 5 



