MIYA, MARCUS AND PHELPS 



If food intake is any criterion, they certainly are stressed, be- 

 cause we have noticed the same thing, 



SCHMIDT: In measuring the effect on conditioned organisms, 

 you tested them after intraperitoneal injection at 32° C, yet 

 rectal temperatures of your mice have been shown to be higher 

 than those of the normal mouse after exposure to cold. I won- 

 der why it is that you decided to investigate the characteris- 

 tics of these organisms at a lower temperature? Why not in- 

 vestigate them at a higher temperature, because this is indeed 

 what you have found in the experimental system. 



MIYA: This was done when we first started into this work. 

 We briefly scanned the literature and most of the reports in- 

 dicated that there was a possible drop in rectal temperatures. 

 We didn't have any suitable temperature measuring equipment 

 at that time, so we decided to approach the problem from a 

 back door; to find out what these organisms would do at dif- 

 ferent temperatures, anticipating this drop. However, we found 

 quite to the contrary that the temperature elevated. 



SCHMIDT: You haven't had the occasion yet to investigate 

 the growth curves or other characteristics of these organisms 

 at one or two degrees above the normal rectal temperature? 



MIYA: No, we haven't. 



REINHARD: In the experiments on the isolates of the "con- 

 ditioned bacteria", how many isolates did you use from each 

 group of mice for determination of oxygen uptake? 



MIYA: These were pooled isolates, 



REINHARD: How did you pool them? 



MIYA: We killed the animals and then opened up the peri- 

 toneal cavities aseptically and just removed the exudate. 



NUNGESTER: Without plating? 



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