MIYA, MARCUS AND PHELPS 



MIYA: They have a slight amount of sawdust in the cage, 



BERRY: This could make a difference. 



MIYA: Our singly caged mice are placed in complete wire 

 cages and do not have any bedding. 



MIRAGLIA: Do these mice lose their tails? 



MIYA: Occasionally you will see a singly caged mouse that 

 will lose its tail completely. 



PREVITE: Is your cage of a standard size, or intentionally 

 smaller because you have only one mouse in it? 



MIYA: The dimensions are approximately 4 in. by 4 in, by 

 7 in. per mouse. 



PREVITE: He has plenty of room. 



BERRY: After these animals have been acclimatized at low 

 temperatures for forty- five days, have you subjected them to 

 an even more acute exposure, say, -20° C, in a deepfreeze, 

 for example. Can they survive a super stress longer than ani- 

 mals that have not been subjected to this long period of acclima- 

 tization? 



MIYA: We haven't done any experiments like that. 



BERRY: This might be of value, because altitude exposed 

 mice that have "acclimatized" are able to withstand a h3T)oxic 

 level for longer periods of time than animals that have not been 

 at simulated altitudes. Possibly these animals that have been 

 maintained at a low temperature, say of 2° C, for some time, 

 may be able to withstand more acute cold stress. If you could 

 show something of this type, I would be a little happier about 

 calling them acclimatized to cold. Your results afford a very 

 good argument for acclimatization, because animals with prior 

 experience at low temperature respond to challenge differently 

 from animals that have never had a previous experience. One 



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