TUNEVALL AND LINDNER 



curring in blood, but in blood only once without being present in 

 the peritoneum. 



The degree of correspondence between the bacterial findings 

 from peritoneum and those from blood is visible from Figure 5a. 

 Full conformity existed in 39 cases, whereas in 9 mice, bacteria 

 were present in peritoneal fluid but not in blood, and in 6 in- 

 stances in blood without being present in peritoneum. Partial con- 

 formity existed in the remaining 7 cases. 



Influence of Hypothermia on the Elimination of Bacteria From the 

 Blood 



In a number of preparatory experiments various numbers of 

 E. coli of strains emanating fron. mouse feces were injected intra- 

 venously into normal mice. After different periods, the blood count 

 of live bacteriawas measured. The results are reported in Figure 6. 

 Regardless of the number of bacteria injected, the elimination 

 was rapid during the first 15 minutes, bringing down the bacterial 

 counts to about one tenth of the numbers found 4 minutes after 

 the injection. In one experiment, where the first count was made 

 only one minute after the injection, the reduction was equally 

 rapid from this time. After the fifteenth minute, the elimination 

 rate was reduced. This varied somewhat between different experi- 

 ments, but always resulted in a complete or almost complete 

 elimination. All animals survived. 



Considering the frequent occurrence in the blood of E, coli 

 after prolonged hypothermia, some other organism not usually 

 found in endogeneous bactermia had to be chosen for the elimin- 

 ation studies on hypothermic mice. Hence, the following experi- 

 ments were performed with Klebsiella pneumoniae in a constant 

 dose of 4 X 10^ bacteria. The following groups, each containir^ 

 5 mice, were studied: normal mice; mice having received pre- 

 medication but not chilled; mice made hypothermic 5 minutes 

 after the injection of bacteria, 2 hours before the injection, 24 

 hours (2 groups), and 48 hours (2 groups) before same injection. 

 The results are presented graphically in Figure 7, 



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