PERITONITIS AND BACTEREMIA IN MICE 



and chill it down there. Then it was cultured the following day. 



MIYA: Is it possible that some of your positive blood cul- 

 tures were just due to the breakdown of your normal tissue 

 barriers before you got the blood? 



TUNEVALL: Maybe this could not be entirely excluded, but 

 we have done some model experiments to see if this treatment 

 of the dead mouse very often results in growing through from 

 the intestine, but we have not the impression that this factor 

 has disturbed the results very much, 



MARCUS: Yesterday, when the speaker spoke about anti- 

 body formation and measurement in rabbits, he spoke about 

 it in rabbits using quantitative antibody estimations by classi- 

 cal methods of determination. He did not consider the possi- 

 bility of formation of non- precipitating antibody, and I think 

 that this is probably valid because most rabbits don't ordinar- 

 ily form too much of this. But you are using mice, and I won- 

 dered if this might not be a significant consideration. I don't 

 know what the system was that you employed for measuringf. 

 the antibody, but I wondered if you had given it any consider- 

 ation at all? 



TUNEVALL: We used the typhoid- H antigen, and for deter- 

 mining the antibody level, we simply used the Widal test, 



MARCUS: One other thing I wanted to ask about. In discussing 

 the mechanism of invasion of the hypothermic mouse, in com- 

 paring it to what occurs in the irradiated animal, there are 

 some interesting possibilities. As far as I know, no one knows 

 the molecular lesions that occur following irradiation which 

 lead to invasion, but there is good evidence at the cellular level 

 of what the lesions and resistance are. The mouse does not have 

 any bactericidal component in its serum, but following irradi- 

 ation there is not a loss of phagocytic capacity, but a loss of 

 intracellular destructive capacity, and I wonder if this is the 

 same type of lesion which might occur in the hypothermic mouse. 



TUNEVALL: I'm sorry, I cannot answer that question, 



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