MIRAGLIA AND BERRY 



19. Walker, D. L., and W. D. Boring. 1958. Factors influencing host- 



virus interactions. Ill, Further studies on the alteration of 

 Coxsackie virus infection in adult mice by environmental 

 temperature. J. Immunol. 80: 39-44. 



20. White, C. 1952. The use of ranks in a test for significance for 



comparing two treatments. Biometrics 8: 33-41, 



21. Wilcoxon, F. 1949. Some rapid approximate statistical pro- 



cedures. American Cyanamid Co., New York. 



22. Wildfilhr, G. 1950. Uber die beeinflussung des Alexin-und 



Opsoningehaltes bei Erkaltungsdisponierten durch Kaltereize, 

 Arch. Hyg. und Bakteriol. 133: 49-58. 



DISCUSSION 



NUNGESTER: Here, again, we have the problem of the hypo- 

 thermic animal apparently secondarily invaded more frequently 

 than an animal kept at the normal ambient temperature. 



MIRAGLIA: Did you say hypothermic animal? 



NUNGESTER: Yes. 



MIRAGLIA: We try to be very careful to avoid this. The normal 

 controls were not hypothermic, but of course when the animal 

 is infected, the temperature does go down. 



BERRY: I would just like to ask an obvious question. Should 

 this be called a secondary infection? If infection implies any 

 disadvantage to the host, I am not sure this is an infection in 

 these mice. It is an interesting observation, however. Dr. Miraglia 

 is attempting to establish coagulase-positive staphylococci in 

 the respiratory tract to see if he can then isolate coagulase- 

 positive staphylococci from the deep tissues. If it can be achieved 



294 



