104 The Lymphocyte and Lymphocytic Tissue 



from the work of Chase, who succeeded in transferring hypersensitivity to 

 tuberculin by cells from peritoneal exudates, lymph nodes, and spleens of 

 tuberculin-sensitive guinea pigs. 22 In 1950 Chase reported the transfer to 

 normal guinea pigs of skin hypersensitivity and anaphylactogenic antibodies 

 to picryl chloride by injection of cells from peritoneal exudates, lymph 

 nodes, and spleens of guinea pigs highly sensitive to picryl chloride. If, in 

 such experiments, the donor animals had been injected with sheep erythro- 

 cytes, hemolysins could be found in the sera of the recipient animals. Ex- 

 tracts of the cell suspension, or cell suspensions which had been frozen and 

 thawed, failed to produce the effect. - :! The transfer of tuberculin hypersen- 

 sitivity has since been confirmed by many other workers. 



In studies of humoral antibody production, Harris, Harris, and Farber in- 

 jected dysentery bacilli into the feet of rabbits, excised the draining popliteal 

 lymph nodes, and transferred suspensions of cells obtained from these nodes 

 into fresh rabbits. Agglutinins to dysentery bacilli appeared in the sera of the 

 recipients in a characteristic pattern. 24 Wager and Chase-"' and Stavitsky 20 

 reported the appearance of diphtheria antitoxin in recipients of cells ob- 

 tained from spleens and lymph nodes of immunized donors. Roberts and 

 Dixon transferred to irradiated recipients lymph node cells (popliteal, 

 mesenteric, and axillary nodes) from donor rabbits immunized over a five- 

 week period with bovine y-globulin or bovine serum albumin. The recip- 

 ients were then injected with radioiodinated bovine y-globulin or with bo- 

 vine serum albumin. The rate of elimination of these proteins from the cir- 

 culation of the recipients was characteristic of a secondary response. The 

 authors calculated that the total homologous antibody synthesized by the 

 transferred cells during the first eight days of the secondary response 

 amounted to approximately two thirds of the wet weight of the transferred 

 cells. 18 



Recently, Sibal and Olson injected bovine serum albumin intravenously 

 into hens and two days thereafter excised the spleens and obtained cell 

 suspensions which were transplanted to the chorioallantoic membrane of 

 embryonated eggs. The transplants were removed after from four to seven 

 days of incubation, and extracts of these were found to contain low titers of 

 antibody to bovine serum albumin, as indicated by adsorption-hemagglutina- 

 tion tests. 27 In experiments with guinea pigs, Rosenberg and collaborators 28 

 gave the donor animals a single intravenous injection of pooled human 

 scrum and hen egg albumin and removed the spleen and lymph nodes at 

 various intervals thereafter. Cells obtained from these tissues were injected 

 intradermal ly into recipient guinea pigs. Evidence of the development of 

 antibody in the recipient was obtained by positive passive cutaneous anaphy- 

 laxis twenty-four hours or more after transfer. 



