106 The Lymphocyte and Lymphocytic Tissue 



suis and .S'. paratyphi, transferred these to two- to five-day-old rabbits, and 



found homologous serum antibodies in 90 per cent of the recipients a few 



days after cell transfer. 1 ' 1 In experiments involving bovine y-globulin or 



bovine serum albumin as the antigen for in vitro incubation with lymph 



node cells, Roberts and Dixon were not able to detect antibody in the sera 



of recipient rabbits unless the recipients were also injected with the anti- 

 o- en 18 



Lymph Node Cell Transfer in Studies of Tissue Transplantation 

 Immunity 



The strong body of evidence on the immunologic potency of lymph node 

 cells has been applied by several workers in the field of tissue transplanta- 

 tion. In studies of immunity to transplantable tumors, Mitchison 36, 37 ob- 

 tained cells from mouse lymph nodes regional to solid tumor homografts and 

 transferred them, either minced or in suspension, to a secondary host. It was 

 found that when the recipient mouse was challenged by a homograft of the 

 same tumor, it destroyed it two or three times more quickly than would 

 otherwise have been the case. The transferred cells had the capacity to confer 

 immunity on the recipient if the nodes were removed from the donor mouse 

 at the time the grafted tumor was undergoing breakdown. Cells from non- 

 regional lymph nodes and from the spleen, as well as serum or whole blood, 

 failed to transfer immunity. Evidence was presented favoring the hypothesis 

 that "the lymph node cells were immunologically activated before transfer, 

 and that they conferred immunity by continuing to function in their host."' 7 

 Homografts of a transplantable sarcoma gave rise to the production of serum 

 antibody which could be detected by its cytotoxic action on the cells of the 

 tumors and also by means of a hemagglutinin test. Following the transfer 

 of regional lymph node cells from mice with such grafts into hosts of the 

 same strain, hemagglutinins could be detected in the host serum. However, 

 the capacity of the cells to transfer hemagglutinin production developed later 

 than the power to transfer increased resistance to grafts. Splenic cells 

 also transferred hemagglutinin production, although to a lesser extent. 

 The conclusion was drawn that the hemagglutinating antibodv is distinct 

 from the antibody effective in protection against homografts. 38 



In the area of skin homografting, Billing-ham, Brent, and Medawar gave 

 mice of one strain skin homografts or injections of leukocytes from mice of 

 a second strain and then transferred fragments or cells of the regional lymph 

 nodes to other mice of the second strain by the intraperitoneal route. The 

 mice that received the lymph node tissue fragments or cells showed height- 

 ened resistance to further skin homografts from the strain used for immuni- 

 zation. The transfer was not effective when contralateral lymph nodes were 



