Role of the Lymphocyte in Antibody Formation 85 



of the immune response in the recipients; and (3) the transfer of cells killed 

 by repeated freezing and thawing or by incubating at 60° C. until trypan 

 blue stains showed dye uptake by over 75 per cent of the cells. Killed cells 

 were transferred to (1) observe the host reaction to the transferred cells and 



% ^X t..—v. ,„ r. • ■» ^ 



*?** '. V *r*r *v 



'! ¥ 





Fig. 7-2. Recipient site 1 day alter transfer of living lymph nodi' cells. Hematoxylin 

 eosin stain, (x 300) 



(2) determine if the prod tut ion of anti-bovine serum albumin needed viable 

 cells. 



RESULTS 



The transferred donor cells were composed of approximately 90 per cent 

 lymphocytes, 8 per cent macrophages and reticuloendothelial cells, and 2 

 per cent plasma cells (Fig. 7-1). 



When live cells were transferred and subsequently sti undated with ami- 



