84 The Lymphocyte and Lymphocytic Tissue 



albumin was observed by studying the elimination of antigen"' 4 and was 

 measured by determining the amount of circulating antibody in the sera of 

 recipients after the antigen was eliminated. 55 



Smears of the donor cells before transfer were stained using Papanicolaou 

 and Wright's stains. The recipients were sacrificed at various times during 

 the experiments, and tissue from the injection sites was fixed in Formalin 



•#« 



i 



Fig. 7-1. Preinjection smear. Lymph nock' cells. Wright's stain. 

 (x 1300) 



for permanent sections. In some experiments performed by Drs. Neil and 

 Dixon,"' 1 the tissue was quick frozen to provide material for immunohisto- 

 chemical study of the sites for antibody, according to the Coon's technique; 56 

 and smears were made from loose cells in the transfer sites. Both hema- 

 toxylin-eosin and pyronine-methyl green stains were used on the fixed 

 material. 



Controls used in the cell transfer studies included (1) the transfer of cells 

 to recipients with no subsequent injections of antigen — for detection of 

 significant residual production of antibody by the cells and passive transfer 

 of antibody; (2) the injection of antigen into x-rayed recipients which did 

 not receive cells — for detection of lack of x-radiation effect on the ablation 



