Cytochemistry of the Lymphocytes 



43 





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Figs 3-37 through 3-42. Histochemistry of sections of lymphoid tissue. 



Fig. 3-37. Demonstration of protein-bound sulfhydryls in the cytoplasm oi the small 

 lymphocytes and stellate reticulum cells in normal human lymph nodes. DDD method 

 with nuclei counterstained with methyl green. (X 1800; reduced 25 per cent) 



Fig. 3-38. Demonstration of acid phosphatase activity in the cytoplasm of lymphocytes 

 and stellate reticulum cells from normal human lymph node. Nuclear staining has been 

 considered to be nonspecific. Gomori acid phosphatase method with prolonged incuba- 

 tion time (16 hours), (x 1800; reduced 25 per cent) 



Fig. 3-39. Alkaline phosphatase is demonstrable in small amounts in the cytoplasm of 

 the lymphocytes and stellate reticulum cells of normal human lymph nodes. Nuclear 

 staining has been considered as nonspecific. Gomori alkaline phosphatase method with 

 prolonged incubation time (16 hours), (x 1800; reduced 25 per cent) 



Fig. 3-40. Localization of beta-glucuronidase in the lymphoid cells of rat spleen. Darker 

 staining cells represent macrophages. Method of Seligman ei at. (x 300; reduced 25 

 per cent) 



Fig. 3-41. Rat lymph node stained for alkaline phosphatase by the Gomori method. 

 Incubation time 14 hours. (X 300; reduced 25 per cent) 



Fig. 3-42. Rat lymph node stained for alkaline phosphatase b\ the Gomori method 

 2 hours following 600 r total body x-radiation. Note the marked decrease in reactivity of 

 the lymphoid cells following radiation. Incubation time 14 hours, (x 300; reduced 25 

 per cent) 



