\ 



/ 



* • 



29 



I 



31 



32 



33 



35 



36 



Figs. 3-28 through 3-36. Histochemistry of leukemic lymphocytes, (x 2000; reduced 25 

 per cent) 



Fig. 3-28. Lymphocyte from chronic lymphocytic leukemia stained by the periodic acid- 

 Schiff method. Note large number of PAS-positive cytoplasmic granules. 



Fig. 3-29. Lymphosarcoma cells stained with the periodic acid-Schiff method revealing 

 a large number of PAS-positive granules in their cytoplasm. 



Fig. 3-30. Lymphoblast (arrow) from acute lymphoblastic leukemia stained by the 

 periodic acid-Schiff method. PAS-positive granules are very small and are generally less 

 numerous than in normal lymphocytes. 



Fig. 3-31. Two lymphocytes from chronic lymphocytic leukemia stained for protein- 

 bound sulfhydryls by the DDD method. Sulfhydryls localize to the cytoplasm and nu- 

 clear sap. 



Fig. 3-32. Two lymphosarcoma cells stained by the DDD method for protein-bound 

 sulfhydryls. Sulfhydryls can be localized to the cytoplasm and nucleoplasm. 



Fig. 3-33. Considerable variations in sulfhydryl content of the lymphosarcoma cells 



40 



