/ 



19 



20 



21 





24 ' 



27 



Figs. 3-19 through 3-27. Histochemistry of normal lymphocytes, (x 2000; reduced 25 

 per cent) 



Fig. 3-19. Normal lymphocyte exhibiting basophilic cytoplasm. Methyl alcohol fixation. 

 Wright's stain. 



Fig. 3-20. Normal lymphocyte exposed to ribonuclease for 10 minutes followed by 

 Wright's stain. Cytoplasmic basophilia has been removed by enzymatic digestion. 



Fig. 3-21. Normal lymphocyte stained by the periodic acid-Schift method. Note the 

 small faintly stained PAS-positive granules in the cytoplasm. 



Fig. 3-22. Normal lymphocyte stained for masked lipids. Small sudanophilic granules 

 can be distinguished in the cytoplasm. Nuclear staining may represent nuclear lipid. 

 Sudan black following unmasking with acetic acid. 



Figs. 3-23 and 3-24. Normal small lymphocytes stained for protein-bound sulfhydryl 

 groups by the I)I)D method of Barrnett and Seligman. Sulfhydryls are localized in the 

 cytoplasm and nucleoplasm while the chromatin is essentialh unstained. 



36 



