482 GENETICS OF SOMATIC CELLS 



Fig. 64. Polyoma hi. 



I 



u*** 



J 9 



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I \\ 



0n Xy Xl 



+ ** 



V 





V 



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* I 



Top "Wet" squash preparation of polyoma III. Quasidiploid state with four 



reciprocal translocations involving chromosomes 1, 2, 3, and 4 at the centromere. Note 

 intact "predominant AV and Y chromosomes. 1450 X. 



Bottom Permanent slide of polyoma III following Conger and Fairchild's 



technique. 221 Slight fuzziness of chromosomes, due to aging of slide temporarily sealed 

 with Kroning cement for several weeks. Making the preparation a permanent one is best 

 done some 72 hours after squashing to eliminate such alterations in chromosomal morpho- 

 logy. 1450 X. 



