534 



APPENDIX 



Let us now work through an example of using all the parts of the system just 

 described as they would apply to an inbred strain and to a new deviant. 



A new inbred strain, NE, was procured and the supply consisted of three sibling 

 pairs. The information given was the parentage of these siblings, their date of birth, 

 the generation of inbreeding and the identification numbers of the individuals. Step- 

 wise the procedure is as follows: 



1 . Check the ear marks of the animals against the information provided to make 

 sure of their identity. 



2. Place each female with her respective brother in a pen labeled NE1, NE2, 

 and NE3. 



3. Make out a cage tag putting on it the information desired. 



4. Make out pedigree cards for each of the females and place in the card file. 



5. Start a pedigree chart with the information that has been provided. 



A new strain is now in business. The pens should be checked frequently to 

 ascertain the continued good health of the animals and whether the females have 

 become pregnant. When litters are born these are immediately recorded on the cage 

 tag. The litters should be weaned about 25-30 days of age. If a new litter is born 

 before that, the older litter may be set out for a few days before it is pedigreed. 

 First litters of any females are not usually used to make up new pairs, but are set aside 

 for experiments or for other purposes. When the second litter is ready to wean, the 

 animals will be recorded in the ledger, new pedigree cards will be made out and new 

 pens put into use. Those animals used as pairs will be recorded on the pedigree chart 

 (figures 80-83). 



Fig. 80. Pedigree card made out for one pair of the new strain just procured. 



