504 GENETICS OF SOMATIC CELLS 



An Introduction to Cell and Tissue Culture. The Staff of the Tissue Culture Course. 

 Cooperstown, New York. Burgess Publishing, Minneapolis, Minnesota. 1949-53. 



1960 Catalogue of the Colorado Serum Company Laboratories, 4950 York Street, Boulder, 

 Colorado. 



Pamphlets on Viral Diagnostic Reagents, Microbiological Cell Culture and Repository, 

 Media for Growth and Maintenance of Cells. Microbiological Associates, Inc., 

 4813 Bethesda Avenue, Bethesda 14, Maryland. 



Difco Manual. Ninth Edition. Difco Laboratories, Inc., Detroit 1, Michigan. 



Reagents, Media, and Cell Lines for Tissue Culture and Virus Propagation. Difco Labora- 

 tories, Inc., Detroit 1, Michigan. 



Listing of Chemicals. Nutritional Biochemicals Corporation, Cleveland, 28, Ohio. 



Products for the Microbiological Laboratory and Diagnostic Reagents. Baltimore Bio- 

 logical Laboratories, 2201 Asquith Street, Baltimore 18, Maryland. 



PREPARATION OF STOCK SOLUTIONS 



It should be remembered that many of the ingredients that make up the current lists of 

 amino acids, vitamins, and salts of currently popular media are readily prepared in advance 

 as 20-50 X concentrates, filtered in convenient aliquots, and stored either frozen or. re- 

 frigerated. 



When diluted proportionally, several liters of balanced salt solutions or medium are 

 prepared at one time. Serum, antibiotics, and other supplements, if desired, can be added 

 prior to sterilization and, in many instances, stored frozen for long periods without ex- 

 hibiting detrimental effects. The use of a magnetic stirrer is helpful in dissolving stubborn 

 mixtures, especially when additional alkaline or acid concentrates appear to have exceeded 

 the limits of previous experience. 



RECOMMENDED INGREDIENTS FOR THE PREPARATION OF SLIDES 



Euparal. Made in England by Flatters and Garnett, Ltd., 309 Oxford Road, Man- 

 chester 13, England. Distributed in the United States by A. H. Thomas Company, 

 Philadelphia, Pa. 



Sealing Compound. Deckglaskitt nach Kronig fur mikroscopie (Cover glass cement 

 according to Kronig, for microscopy). Riedel-de Haen Ag.AG., Seelze-Hanover, Germany. 

 (Minimal order of $10.00.) 



Colchicine. U.S. P. Abbott Laboratories, Chemical Sales Division, North Chicago, 

 Illinois. 



Orcein. (State natural or synthetic.) George T. Gurr, Ltd., London, S.W.6, England. 

 Canada: ESBE Laboratory Supplies, Toronto, Canada. 



Carmine. Fisher Scientific Supply Co., New York, N.Y. The Matheson Company, 

 Inc., East Rutherford, New Jersey. 



Cover Glasses. (Specify Gold Seal, thinness 0, in \ ounce package, cut to desired sizes.) 

 (For cytology tubes, 10 x 45 mm.) 



IX Detergent. For washing tissue culture glassware. Linbro Chemical Co., Inc., 

 New Haven, Connecticut. (Available from local laboratory supply house.) 



CULTURE TUBES FOR CYTOLOGIC PREPARATIONS 



For use with cover glasses up to 10 mm. wide by 45 mm. long 



Require only 1-ly ml. of medium. Useful for replicate trials and chemical dilution 

 series. Cover glass-attached cells available for general morphologic procedures, wet or 

 dry squash preparations for chromosomal analyses, exposure to tritiated purine and pyrimi- 

 dine analogs, as well as other applications when a C0 2 environment is essential for proper 



