564 APPENDIX 



are anesthetized with ether. A small midline incision is made in the ventral body 

 wall and one horn of the uterus exposed. The desired number of ova to be transferred 

 are drawn up into a pipette with a minimal amount of saline. After an opening has 

 been made in the recipient horn with a hypodermic needle, the pipette containing 

 the ova is inserted, pointing cephalad, and the ova are released into the horn. It is 

 important to inject as little fluid as possible and to avoid injecting any air. The opening 

 in the uterine horn will close without further treatment. The incision is closed with 

 black silk and the female is isolated. A daily check is made of the animal until she 

 produces her litter. 



Various alterations in the technique have been employed by other workers. 351 - 

 352. 827. 828 y^g reac Jer i s referred to the cited articles for these. 



