76 



INTRODUCTION TO CYTOLOGY 



of the Golgi material to the opposite end of the cell. The theory that 

 the Golgi material is intimately concerned in the elaboration of secre- 

 tions was placed on a firm foundation by the researches of Nassonow 

 and of Bowen. It had long been known that the secretion in such cells 

 appears in the form of small droplets or as small granules about which 

 fluid accumulates (see Renaut, 1907, 1911). Parat (1928) found the 

 fluid to be stainable with neutral red. It was clearly shown by Nassonow, 

 Bowen, and others that these secretory droplets arise in the Golgi region. 

 In intestinal goblet cells, for example, the secretion first appears in the 

 form of minute granules or droplets along the strands of the basket-like 



\ :\ 



5 



i.s 



1 ' ^ iS 4 6" 



Fig 37. — The relation of the Golgi apparatus to secretion. 1-5, stages in the forma- 

 tion and accumulation of secretory droplets to form a typical "goblet cell" in the intestinal 

 epithelium of Molge pyrrhogastra. 6, individual secretory granules with attached bits of 

 Golgi material in the pancreas of Cryptobranchus. (After Bowen, 1924a.) 



Golgi network. The droplets pass toward the distal region of the cell, 

 where they collect in large masses and complete their growth. As they 

 move away from the net, each is seen to have a small chromophilic 

 cap or girdle (Fig. 37, 6). 



The principal interpretations which have been placed upon such 

 observations may be outlined as follows. According to Nassonow and 

 Bowen, the Golgi material in such cells is a definitely formed system with 

 a reticulate structure like that illustrated in Fig. 37, and its special 

 function is to produce the secretion. Whether it has a part in the actual 

 synthesis of the secretion (Bowen) or only acts to concentrate a secretion 

 elaborated elsewhere (Nassonow) is uncertain. The latter alternative 

 would seem to be favored by the "secretion" of injected trypan blue, 



