130 Betty L. Rubin, R. T. Dorfman and G. Pincus 



and Sandulesco, 1936), the digitonin-non-precipitable (or a-) 

 fraction of the ketonic extracts was obtained by the method 

 of Butt, Henly, and Morris (1948). This fraction was dis- 

 solved in benzene and analysed by a quantitative paper 

 chromatographic technique which has been described in 



Mept. PG. 

 24 hrs 

 c^-y^ztomc extract 



ll-K-£tio 

 ll-h-And. 



tlutc, or 



Different Aliq. 



M&pt.-P6. 



72hr5. 



■Res 



il-K-Etio. 

 Il-K-And. 



Recovery 

 l^no^f) hetomc 



Cpd5=c. 90/" 

 Residue = c. 10/- 



sz 



£|-|o + E late, oxidize 48 hrs. C 

 A9(")-Etio pcrbenzoLc acid. 

 Mgpt-P6. 4dbrB. 



9, II - Epoxide 



EtLO. 



^ 



Androst.+A^^"^- £lute, oxldiig 5 hrs. c 

 AndrosL perbcnzoic acid 



Wept.-PG. 24 hrs. 



9, 1 1 - Epoxide 

 Androst. 



ELute 



Mept-pCS 

 J8 hrs 



/^^ '^■^-androstene-IT-one 



5/6-Chloro-A^- 



^ndrD6tene-|7-one 



Andro5t^A^^"^-Androst. 

 E 



lord 



Fig. 3. Scheme for quantitative paper chromatography of 

 a-ketonic urine extracts. 



detail (Rubin, Dorfman and Pincus, 1954). A brief summary 

 of the analytical procedure is shown in Fig. 3. An aliquot of 

 the extract is chromatographed in the heptane-propylene 

 glycol system, giving discrete zones of androsterone and 

 8etiocholan-3a-ol-17-one. The materials whose mobilities on 

 paper are more rapid than that of androsterone (botli effluent 

 material and material eluted from the paper strip distal to tlie 

 androsterone zone) are rechromatographed in the hei)tane- 



