740 Experiments 



resolved then to inject into a healthy dog blood which had been 

 greatly superoxygenated. I did so in the following experiments. 



Experiment CCCI. June 30. Defibrinated dog blood, shaken in 

 the apparatus pictured in Figure 45, under a pressure of 10 atmos- 

 pheres of air with 65% of oxygen. Next the excess of gas was expelled 

 by whirling the flask containing the blood at the end of a cord, like 

 a sling. It then contained 24 volumes of oxygen per 100 volumes of 

 blood. I injected 200 cc. of it into the femoral vein of a female dog 

 weighing 6 kilos. 



No symptom, not even apparent discomfort. 



Experiment CCCII. July 23. Defibrinated dog blood; treated like 

 the preceding at 10 superoxygenated atmospheres; it contained 34 

 volumes of oxygen. 



I bled a little dog weighing 1640 gm. from the carotid; I took 

 from him 20 cc. of very red blood (this blood clotted with extraor- 

 dinary rapidity), containing only 7.3% of oxygen, with 33% of car- 

 bonic acid; the arterial pressure was 13 cm. 



I injected into his jugular vein 35 cc. of the blood supersaturated 

 with oxygen, which had been whirled like a sling. 



No effect. 



Experiment CCC1II. August 10. Defibrinated dog blood, treated 

 as above, at 10 superoxygenated atmospheres; contained 33 volumes 

 of oxygen. 



Little dog weighing 2085 gm.; rectal temperature 36°; pulse 160; 

 respiratory rate 50. I took 100 cc. of blood from him; he became very 

 weak; his temperature fell to 34.5°; pulse 128; respiratory rate 30. 



I then injected into his jugular 110 cc. of superoxygenated blood; 

 immediately the animal revives, and when put down on the floor, 

 seems only a little weak. 



No after-effect. 



And so in conditions of compression, that is, oxygen saturation, 

 similar to and even greater than those which caused death, the 

 blood acquired no dangerous quality, and can be substituted safely, 

 in a very great proportion (1/19 of the weight of the body) for 

 the blood of another animal. I must add that the agitation in 

 compressed oxygen had lasted only a very short time, less than 

 one hour. We shall see in Subchapter III of Chapter VI other 

 experiments made from another point of view with blood shaken 

 for several hours with compressed oxygen. 



But let us come to the description of the convulsive attack itself. 

 It is truly curious and terrifying. 



Let us take a case of average intensity. When the animal is 

 taken from the apparatus, it is generally in severe tonic con- 

 vulsions; the four feet are stiff, the trunk is bent back or a little to 



