838 Experiments 



A. In the air, covered with a paper cone. 



B and B'. In 15 atmospheres of superoxygenated air. 



March 5. Decompression. 



A. Smells rather strong and is covered with mold which forms 

 a stopper; very acid. 



B and B'. No odor; no mold; acid. 



I put B and B' each in a glass with 10 grams of cooked white of 

 egg which has been cut into pieces; the glasses, which are of the 

 same size, are next filled with water acidulated with hydrochloric 

 acid. 



I take 2 grams of powdered pepsin, place them in 5 cc. of dis- 

 tilled water, and add 10 grams of the cooked white of egg, and the 

 same quantity of acidulated water. 



The three glasses are placed in the drying oven at 38 degrees. 



March 8. There remain in each glass 2 grams of insipid white of 

 egg. 



So pepsin, after being subjected to the effect of oxygen at high 

 tension, behaved absolutely as before. 



3. Inversive Ferment of Yeast. 



The ferment which I used, like the myrosine and emulsine to be 

 discussed in the following sections, had been prepared by M 

 Schiitzenberger, then head of the laboratory of chemical research 

 of the Faculty of Sciences. 



Experiment CCCCLXXV (2). February 16. Placed 5 cc. of liquid 

 in three tubes drawn out in the flame. 



A. In open air, covered by a paper cone. 



B and B\ At 15 atmospheres of superoxygenated air. 



March 5. Decompression; I close the three tubes with the flame; 

 but first I make sure that the ferment in tubes A and B transforms 

 cane sugar rapidly into glucose. 



March 15. Placed in the drying oven, in which the temperature 

 varies from 25 to 40 degrees. 



March 25. Withdrawn from the drying oven; A, B, and B' ai*e still 

 potent. 



May 17. A is alkaline and foul; has no potency. 



B and B' are acid, have no odor, and have kept their power. 



4. Myrosin. 



Experiment CCCCLXXV I. February 16; in 3 tubes drawn out in 

 the flame; liquid to a height of 5 cm. 



A. Free air, covered with a paper cone. 



B and B'. At 15 atmospheres of superoxygenated air. 



March 5. Decompressed; A and B act perfectly upon potash my- 

 ronate to give essence of mustard. 



March 15. Placed in the drying oven, from 25 to 40 degrees. 



March 25. Taken from the drying oven; A and B are still potent. 



May 17. A, B, and B' are still potent, but the last two have more 

 energetic action. 



