Fermentations by Organisms 817 



B. Blood. 



Experiment CCCCXIV. June 9. Defibrinated dog's blood. 



A. 30 cc. placed in a flask at normal pressure. 



B. 30 cc. in another flask closed by a cork with a hole. Placed in 

 the iron apparatus and taken to 12 superoxygenated atmospheres. 



The pressure falls in the days following, and cannot be kept above 

 8 atmospheres. 



June 13. B is decompressed and sealed. 

 June 18. A. Horrible odor; B. slight odor. 



Experiment CCCCXV. June 19; 1874. Dog blood, fresh, defibri- 

 nated. 



A. In a corked flask, ordinary air. 



B. Flask closed by a stopper of cork with a hole, subjected after 

 agitation to 20 superoxygenated atmospheres, equivalent to 88 atmos- 

 pheres of air. 



June 24. A. Smells very bad. 



B. Decompressed, no odor, is translucent, as if varnished; I seal 

 the orifice of the flask with wax. 



July 6. A. Wrinkled layer on the surface; repulsive odor. No 

 globules visible; vibriones with a shiny terminal point, quite numer- 

 ous, and also motionless bacilli; crystals of hemoglobin. 



B. The blood has become slightly turbid; no white layer on the 

 surface; strange odor, very slightly putrid. Blood corpuscles pink and 

 extraordinarily pale; no crystals; some few vibriones with shiny point. 



July 12. B. Still no putrid odor. 



The stopper is merely put back on B, without being sealed again. 

 In the following months, it is opened frequently and closed without 

 precaution; the stopper even happens to fall on the floor and is put 

 back without any care. Nevertheless the putrid odor does not appear 

 clearly. 



January 16, 1875. Presented to the Society of Biology. 



A. Is horribly putrid. 



B. Can be sniffed without disgust, but does have a slight odor. 



Experiment CCCCXV I. July 21. Dog blood, defibrinated. 

 In equal quantities in: 



A. Matrass closed with a cork stopper. 



B. Similar matrass drawn out in the flame; it is subjected to 15 

 superoxygenated atmospheres. 



July 22. A. Begins to smell bad. 



B. Pressure has fallen; I take B out and shake it to moisten the 

 walls of the matrass; then I take it to 8V2 superoxygenated atmos- 

 pheres. 



July 23. Raised to 12 atmospheres. 



July 24. Raised to 15 atmospheres. 



July 30. A. Smells horribly bad. 



B. Still 14 atmospheres; decompressed; no odor; closed the pointed 

 end of the matrass with the flame. 



A drop of this blood, examined through the microscope, shows no 

 corpuscles; it looks as if it were varnished. 



