Fermentations by Organisms 821 



February 3. Decompressed B and closed the hole with burning 

 wax. A and B have the same appearance. 



May 22. A. The stopper blows out when I open the flask. Very 

 strong butyric odor; very acid reaction. Numerous very active vib- 

 riones, some of which are oval and wide, are to be seen in it. 



B. The stopper does not blow out; very slight butyric odor, very 

 acid reaction. A few bacillus vibriones, very small and active. 



Experiment CCCCXXIV. May 22. Temperature 18°. Boiled milk, 

 placed in four flasks well-washed with hot alkaline water. 



A, A', two flasks well corked and sealed. 



B, B', two flasks, closed with a cork stopper pierced by a hole, 

 taken to 10 atmospheres of air with 70% of oxygen, which is equiv- 

 alent to 35 atmospheres of air. 



May 24. Decompressed B and B' and closed the holes with melted 

 wax. 



The four flasks appear curded to the same degree. 



Experiment CCCCXXV. May 26. Boiled milk, with the addition of 

 water alkalinized by carbonate of soda. 



A, A'. Two flasks are closed and sealed. 



B, B'. Two others, whose stoppers are pierced by a hole, are 

 placed in the cylindrical glass apparatus under a pressure of 10 atmos- 

 pheres of air with 70% of oxygen, or about 35 atmospheres of air. 



June 1. AA' is partly coagulated. 



BB' is hardly coagulated at all. 



June 3. Decompressed BB' and closed the holes with melted wax. 



The liquid is less clearly coagulated in BB' than in AA'. 



June 26. BB' are less clearly coagulated than AA'. 



BB' are neutral or hardly acid. 



AA' are extremely acid. 



Experiment CCCCXXVI. August 7, 1874. Boiled milk, placed in 

 two matrasses, in which the liquid occupies only a small part. 



A, closed with a stopper of new and well heated cork. 



B, drawn out in a flame, except a little hole in the extremity; 

 taken to and kept at a pressure of between 8 and 12 superoxygenated 

 atmospheres. 



August 17. A, yellowish clot with mold; foul. 

 B, decompressed, closed with a flame; white clot. 

 January 18, 1875. A, yellowish mass with deep yellow skin. Smells 

 bad; alkaline reaction. 



B, very white and very clean clot; has no bad odor. 



Experiment CCCCXXVII. August 7, 1874. Boiled milk, with the 

 addition of alkalinized water; arranged as in the preceding experi- 

 ment; one of the matrasses, B, placed beside the one of the experi- 

 ment above. 



August 17, at decompression, same difference in the general 

 appearance. 



January 18, 1875. A, foul; yellowish with a yellow skin; alkaline. 



B, fresh odor, sourish; white, clean clot; reaction quite acid. 



