824 Experiments 



August 15. A, completely turbid, very alkaline, horribly foul. 



B, turbid, fairly alkaline, not quite so foul. 



C, slightly turbid, a little alkaline, begins to smell bad. 



Experiment CCCCXXXIV. May 13. Fresh urine, very acid, in 

 two similar flasks. 



A, corked, at normal pressure; 



B, taken to 10 atmospheres of superoxygenated air. 

 May 18. A, turbid, neutral. 



B, clear, acid. 



Experiment CCCCXXXV. June 19. Mixture of fresh urine and 

 urine already spoiled. 



A, flask with a stopper. 



B, flask with a cork stopper with a hole, taken to 20 atmospheres 

 of superoxygenated air, corresponding to 88 atmospheres of air. 



June 24. A, turbid, smells bad; I close the hole in the stopper 

 with wax. 



B, decompressed; clear, no odor. 



July 6. A, strong odor; turbid; a film on the surface, in which 

 there are myriads of moving organisms and rounded crystals. Strongly 

 alkaline; to acidify a certain quantity, it is necessary to add 4 drops of 

 sulphuric acid. 



B, no odor; turbid; film; moving protozoa, but no crystals. Not 

 very alkaline; a single drop of sulphuric acid acidifies the same 

 quantity as in A. 



July 30. A is horribly foul and very alkaline; B, which had the 

 stopper replaced without care, has no odor and is not very alkaline. 



However the two urines give, by the Yvon method, the same 

 quantity of nitrogen (3.5 to 3.7 per cubic 'centimeter) . 



Experiment CCCCXXXVI. July 21, 1874. Fresh urine, in equal 

 quantities in two equal matrasses: 



A, closed with a cork stopper; 



B, drawn out in a flame, with a tiny orifice. Placed at 15 super- 

 oxygenated atmospheres; matrass shaken. 



July 30. A, turbid, very bad odor. 



B, clear and has no odor. While I was trying to close the matrass 

 with a flame, it broke; I decanted the urine into a similar matrass 

 which also broke, then finally into a closed tube well washed with 

 boiling water, and then closed with a flame. 



In the following months, the turbidness in A keeps increasing; 

 foul odor; color deeper and deeper. 



On the contrary, B remains limpid and pale in color. 



January 16, 1875. Presented to the Society of Biology. A, very 

 dark-colored, turbid, foul; B, clear, with a slight flaky deposit. 



January 18. A, dark-colored, turbid, foul, very alkaline. Analysis 

 by the Yvon process gives 5.8 cc. of nitrogen for 1 cc. of urine; but 

 by the Grc'hant process we get only 2 cc. of nitrogen, that is, 0.5 

 centigrams of urea; that is because the Yvon process includes the 

 carbonate of ammonia. 



