Action of Carbonic Acid 903 



4:50. The heart is still beating; no respiration for about 10 min- 

 utes. 



Drew 50 cc. of blood from the left heart, not very red . . . . B 



5 o'clock. The heart has not been beating for several minutes; 

 drew 50 cc. of very dark blood from the right heart . . . . C 



5:15. The air of the bag contains C0 2 45.4; CL 39; this composi- 

 tion could not have changed perceptibly since death, by endosmosis, 

 for at 6:45, I find in it 44.6% of carbonic acid. 



A. (right heart; breathing oxygen for 5 hrs. 10 min.) contains 

 O, 16.6; CO, 101.4. 



B. (left heart; 2 39; CO, 45.4) contains O. 10.8; CO 116.6. 



C. (right heart; O, 39; CO, 45.4) contains O, 0.7; CO. 120.4. 



Experiment DCXII. March 15. Temperature 13°. Young bitch, 

 weighing 8.5 kilos. 



Vaginal temperature 39°. 



2:25. Caused to breathe through the trachea into the bag contain- 

 ing 40 liters of air with 83% of oxygen. 



Respirations extremely rapid. 



2:30. Took 25 cc. of arterial blood from the carotid; pulse then 

 was 100; the blood is extremely red .... A 



2:55. 72 deeper respirations; vaginal temperature 36.1°. 



3:05. Took air from the bag . . . . x 



3:07. 60 respirations, quite deep; temperature 35°; took 25 cc. of 

 arterial blood . . . . B 



4:04. 56 respirations; temperature 31°; air from the bag . . . . y 



4:06. 25 cc. of blood, very red . . . . C 



4:20. Respirations 44; pulse 51; temperature 30°. 



4:50. Respirations 28; pulse 32; urine. 



5:15. Respirations 20; pulse 24; temperature 27°. 



5:25. Respirations 16; pulse 16; temperature 26.5°; one eye insen- 

 sible; still a little sensitivity in the other. 



5:32. Took 15 cc. of carotid blood, very red . . . . D 



5:38. Temperature 25°; respiration in the bag stopped, sample of 

 the air taken . . . . z 



After a few minutes, the animal, which still lacks eye sensitiv- 

 ity, is seized by rigor in the legs and neck, with a certain uneasiness 

 in the limbs; it is unfastened. It then makes slow and languid move- 

 ments like those of a marmot waking up. 



6:20. Animal calm; respirations 24; pulse 32; temperature 25°. I 

 draw 25 cc. of very red blood, then I take out the tracheal tube . . . E 



The next day, the animal is perfectly recovered; its vaginal tem- 

 perature has risen to 40°. Survives. 



Summary of the experiment: O, 



Blood A (83% of oxygen) __^ 24.7 



Blood B (air x: O^ 71.6; CO, 13.3) 23.4 



Blood C (air y: 3 61; CO, 21.5) 22.6 



Blood D (air z: O, 55; CO, 29.6) 20.3 



Blood E (40 minutes after, breathing free air) 23.0 40.6 25' 



