— 182 — 



40 e. e. of each filtrate. To dissolve the molybdenum phosphate formed 

 were reqiiired : 



24.95 e. e. of " 5 NaOH, corresponding to 0.01379 % phospho- 

 rus in filtrate A ; 



15.9 e. e. of " ,-, NaOH, corresponding to 0.00S79 "'o phosphoriis 

 in filtrate C. 



Results an increase of 0.00500 grms. phosphorus in 100 e. e. of fil- 

 trate A. 



E X p e r i m e n t No. 5. 



An other brain, weighing 58 grms., was prepared as in previous 

 experiments. On the brain paste obtained the following researches were 

 made : 



a. 5 grms. of brain paste gave 0.9532 grms. of dry residue, i. e. 

 19.06 O/o. The dry residue incinerated left 0.0694 grms. of ash, i, é. 

 1.39%; and consequently 0.8838 grms. of organic matters, i. e. 17.67 «/o. 



b. Total nitrogen estimation. 1.0552 grms. of brain paste 

 required, according to Kjeldahl, 12.45 e. e. of " j,, HCl, corresponding 

 to 1.65 0/0 nitrogen, and 



1.7386 grms. of brain paste required 20.15 ce. of "',„ HCl. corre- 

 sponding to 1.62 o/q nitrogen. 



Hence, in average, the brain contains 1.63 o/o total nitrogen. 



e. Phosphorus estimation. 1 .0396 grms. of brain paste requi- 

 red, according to Neumann , 12.25 e. e. of " /- NaOH, which corre- 

 spond to 0.260 o o phosphorus, and 



1.5400 grms. of brain paste required 18 ce. of "/j NaOH, which 

 correspond to 0.258 o/o phosphorus. 



Therefore, the brain contains, in average, 0.259 o/o phosphorus. 



d. Research on ferme nts. To this end the following two mix- 

 tures were made : 



A : 24 grms. of brain paste were mixed with 240 e. e of chloro- 

 form water adding besides 2.4 e e of pure chloroform (Main-research). 



C : 24 grms. of brain paste were mixed with 240 e e. of water 

 and heated to boiling point. After cooling 2.4 e. e. of pure chloroform 

 were added (Control-research). 



Both mixtures were left to incubate at 37" for 96 hours. 



On the filtrates A and C, obtained as before, the following deter- 

 minations were made : 



1. 40 e. e. of filtrate A gave 0.2176 grms. of dry residue, i. e. 

 3.190/00; 



