— 183 — 



40 e. e. of filtrate C gave 0.0936 grms. of dry residue, i. e. 2.34 7oo- 



The residiies incinerated left : 



0.0412 grms. of ash, i. e. 1.03 o'oo, in filtrate A; 



0.0388 grms. of ash, i. e. 0.97 » oo in filtrate C ; 



and consequently : 



0.0864 grms. of organic matters, i. e. 2.16 Oyo, in filtrate A ; 



0.0548 grms. of organic matters, i. e. 1.37 "oo, in filtrate C. 



2. Nitrogen estimation. The nitrogen was estimated in 20 

 e. e. of each filtrate. To neutralise the ammonia resulting were required: 



3.85 e. e. of " /,,j HCl , which correspond to 0.0269 °/o nitrogen 

 in filtrate A; 



2.5 e. e. of " ,\o HCl, wich correspond to 0.0175 "'« nitrogen in 

 filtrate C. 



Results an increase of 0.0094 grms. nitrogen in 100 e. e. of fil- 

 trate A. 



3. Furine nitrogen estimation. The silver compound, pre- 

 cipitated in 100 e. e. of filtrate A, required, according to Kjfdahl. 1.75 

 e. e. of " /i„ HCl, which correspond to 0.00245 o'o purine nitrogen. 



The ammoniacal silver nitrate yielded no precipitate in the filtrate 

 of the control-research. 



4. Phosphorus estimation. To dissolve the molybdenum pho- 

 sphate precipitated from 40 e. e. of each filtrate, according to Nf.umann, 

 were required : 



18.5 e. e. of " '. NaOH, which correspond to 0.01023 per cent pho- 

 sphorus in filtrate A ; 



12.5 e. e. of "'5 NaOH, which correspond to 0.00691 per cent pho- 

 sphorus in filtrate C. 



Results an increase of 0.00332 grms. of phosphorus in 100 e. e. of 

 filtrate A. 



E X p e r i m e n t No. 6. 



The brain of a dog freshly killed was pounded to a honioge- 

 neous paste. It weighed 52 grms. 



Research on ferments. With the brain paste obtained the 

 following two mixtures were made : 



A : 24 grms. of brain paste were mixed with 240 e. e. of chlo- 

 roform water adding besides 2.4 e. e. of pure chloroform (Main-research). 



C : 24 grms. of brain paste were mixed with 240 e. e. of water 

 and heated to boiling point. When cool 2.4 e. e. of pure chloroform 

 were ad.ded (Control-research). 



