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Research on ferments. Two mixtures were made as follows: 



A : 24 grms. of brain paste were mixed with 240 e. e. of chloro- 

 form water adding besides 2.4 e. e. of pure chloroform (Main-research). 



C : 24 grms. of brain paste were mixed with 240 e. e. of water 

 and heated to boiling point ; after cooling 2.4 e. e. of pure chloroform 

 were added (Control-research). 



Both mixtures were put together in the incubator at 37» and left 

 there for 96 hours shaking them now and then. 



On the filtrates A and C, obtained in the same way as in experi- 

 ment No. 1, the following determinations were made : 



1. 40 e. e. of filtrate A gave 0.1232 grms. of dry residue i. e. 

 3.08 o/oo; 



40 e. e. of filtrate C gave 0.0896 grms. of dry residue i. e. 2.24° oc 



The residues incinerated left : 



0.0396 grms. of ash i. e. 0.99» oo i" filtrate A ; 



0.0372 grms. of ash i. e. 0.93 " ,o in filtrate C; 



and consequently : 



0.0836 grms. organic matters i. e. 2.09 " oo •" filtrate A ; 



0.0524 grms. organic matters i. e. 1.31 '^-.^o in filtrate C. 



2. Ni froge n estimation. In 20 e. e. of each filtrate the ni- 

 trogen \xas estimated. 



To neutralise the ammonia resulting, according to Kjeldahl, were 

 required : 



3.3 e. e. of "io HCl, corresponding to 0.0231 o/o nitrogen, in filtrate A; 



2.2 e. e. of "/in HCl, corresponding to 0.0150 o/o nitrogen, in filtrate C. 



Results an increase of 0.0081 grms. nitrogen in 100 e. e. of filtrate 

 A, corresponding to 10 grms. of brain. 



3. Furine nitrogen estima tion. The Silver compound pre- 

 cipitated in 100 e. e. of filtrate A required, according to Kjeldahl, 1.7 

 e, e. of "/i„ HCl, which correspond to 0.00238 "/o purine nitrogen. 



No precipitate was yielded by the ammoniacal Silver nitrate in the 

 filtrate of the control-research. 



4. Phosphorus estimation. To dissolve the precipitate of mo- 

 lybdenum phosphate formed in 40 e. e. of each filtrate, according to 

 Neumann, were required : 



17.7 e. e. of "/- NaOH, corresponding to 0.00978^0 phosphorus 

 in filtrate A ; 



12.1 e. e. of "/^ NaOH, corresponding to 0.00669 «o phosphorus 

 in filtrate C. 



Results an increase of 0.00309 grms. phosphorus in 100 e. e. of 

 filtrate A. 



