186 



Experiment No. 8. 



An other brain was prepared as before. It weighed 52 grms. 



Research on ferments. Por this purpose the following two 

 mixtures were made : 



A : 24 grms. of brain paste were mixed with 240 e. e. of chloro- 

 form water adding besides 2.4 e. e. of pure chioroform (Main-research). 



C : 24 grms. of brain paste were mixed with 240 e. e. of water 

 and heated to boiling point. When cool 2.4 e. e. of pure chioroform 

 were added (Control-research). 



Both mixtures were put together in the incubator at 37° and left 

 there for % hours. 



On the filtrates A and C, obtained as before, the following deter- 

 minations were made : 



1. 40 e. e. of filtrate A gave 0.1112 grms., i. e. 2.78 «'oo, of dry 

 residue ; 



40 e. e. of filtrate C gave 0.0820 grms., i. e. 2.05 » oc of dry re- 

 sidue, 



which incinerated left : 



0.0424 grms. i. e. 1.06 °/oo ash in filtrate A; 



0.0396 gtms. i. e. 0.99 "oo ash in filtrate C; 



and consequently : 



0.0688 grms. i. e. 1.72 o/qo organic matters in filtrate A; 



0.0424 grms. i. e. 1.06 o/oo organic matters in filtrate C. 



2. Nitrogen estimation. To neutralise the ammonia distil- 

 led over from 20 e. e. were required : 



3.7 e. e. of "/io HCl, corresponding to 0.0259 "/o nitrogen, in fil- 

 trate A; 



2.5 e. e, of "/i,j HCl, corresponding to 0.0175 ''/o nitrogen, in fil- 

 trate C. 



Results an increase of 0.0084 grms. nitrogen in 100 e. e. of filtrate 

 A, corresponding to 10 grms. of brain. 



3. Furine nitrogen estimation. The precipitate of the Sil- 

 ver compound , obtained from 100 e. e. of filtrate A was kjeldahlized. 



1.85 e. e. of " /ji,j HCl were required to neutralise the ammonia 

 resulting, which correspond to 0.00259 °:o purine nitrogen. 



No purine bases were precipitated in the filtrate of the control 

 research. 



4. Phosphorus estimation. To dissolve the precipitate of 

 molybdenum phosphate resulting from 40 e. e. were required: 



